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A variety of techniques have been developed to isolate genomic DNA from the different biological sources. The most popular methods are the solid phase based extraction methods. In these methods, the biological samples are lysed in an apropriate buffer that allows the release DNA is selectively adsorbed on the solid phase. This is followed by washing step which washes away the non-specifically adsorded components (such as proteins, lipids and PCR inhibitors). Fianally the adsorbed DNA is eluted by using an apropriate elution buffer. The genomic DNA extraction kits are commercial available from the different vendors. However, the high cost and low purity in the most of commercial available DNA extraction kits had a significant disadvantage to the customers. An incomplete removal of PCR inhibitors and some PCR inhibitors may still be present even after using DNA extraction kit, which often cause low sensitivity or false negative results on the PCR-based nucleic acid testing.


 

GX Genomic DNA Kits for Cell or Blood contain all the reagent components needed to purify the genomic DNA from mammalian cells and blood. The cells are first lysed in the LN buffer that allows to lyse the cell membrance. This is followed by centrifugation and discarding the supernatent. The pellet is further lysed in the GL buffer which allows the released DNA to selectively adsorb the silica membrane. After washing the membrane two times, all the non-specifically adsorbed components (such as PCR inhibitors) are eliminated. The adsorbed DNA is finally eluted using the elution buffer A.

GX Cell-Free DNA Kit contains all the reagent components needed to purify the genomic DNA from serum or plasma or other body fluid which contain DNA. Cell-free samples are first diluted in the LN buffer. This is followed by centifugation and discarding the supernatent. The pellet is further lysed in GL buffer which allows the released DNA to selectively adsorb the silica membrance. After washing the membrance two times, all the non-specifically adsorbed components (such as PCR inhibitors) are eliminated. The adsorbed DNA is finally eluted using Elution Buffer A.

The new technology allows to extraction a large volume of mammalian cells, blood and cell-free samples (serum or plasma) in one column, and the final eluted DNA is more pure since the most PCR inhibitors are eliminated in the inital pelleting procedure. The new DNA extraction method is guaranteed the purity and the integrity of the extracted DNA in cost-effect manner. In addition after inital pelleting and resuspending the pellet with 200uL of PBS, the rest extraction procedure can be fully automatable on QIAcube.

 

Purification Procedure for GX Genomic DNA Extraction Kit

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